AI models for designing DNA.
GeneLoop turns a researcher’s instructions into DNA designs they can edit and check before laboratory work.
What we do.
Illustration of what Helicase 1, a model that takes DNA and text in and gives DNA and text out, is designed to do. You ask, “Make a new DNA construct that knocks out p53 in human cells.” It thinks about the gene and the guide, then writes a Cas9 and guide plasmid, 9,174 bases from U6 promoter, Guide and its scaffold, CBh promoter: CMV enhancer, chicken β-actin promoter and hybrid intron, SpCas9 with a 3×FLAG tag and nuclear localisation signals, T2A and puromycin resistance, bGH polyA signal, f1 origin, Ampicillin resistance and its promoter, Plasmid origin of replication, as raw DNA, and GeneLoop’s verifier checks the finished file. No model wrote the sequence.
Make a new DNA construct that knocks out p53 in human cells.
Here is pTP53-KO, 9,174 bases, written as DNA.
pTP53-KO carries the TP53 guide, SpCas9 and a puromycin marker. Check the guide for off-target sites before you order it, then transfect, select with puromycin and confirm the edit by sequencing.
Checks the fileGeneLoop verifier
It reads the finished sequence, not the plan.
- Guide targets TP53 beside an NGG PAM
- Cas9, T2A and puromycin read as one frame
- Ampicillin gene intact
pTP53-KO.gb is ready to review before you order DNA.
An illustration of what Helicase 1 is designed to do: think, then answer in text and DNA. No model wrote the reasoning or the sequence. The sequence is Addgene’s PX459 V2.0 (plasmid #62988) with a TP53 guide in it: the top-ranked SpCas9 guide in the first coding exon of NM_000546.6, by GeneLoop’s guide scoring. The reasoning and the checks are worked out from that sequence.
Helicase is a genome language model.
DNA and text go in. Edited DNA and text come out.
Explore the research“Replace position 5 with G.”
What you can do with GeneLoop.
Design a DNA construct
Start with a DNA template, the sequence to add and the target organism. Get an edited sequence file to inspect in GeneLoop.
Verify before you order
Check that DNA parts join correctly and coding regions remain intact. Review errors and unresolved questions before ordering DNA.
Plan lab automationComing soon
Planned: turn a design workflow into an Opentrons liquid-handling protocol for review and testing before a robot run.
Review every change before it reaches the lab.
GeneLoop returns an edit as a before-and-after map: which annotations were added, removed or disrupted, and the net change in length. You check the construct before you order DNA.
See the platformWhere Helicase is going.
Helicase is designed to get better the more it is used.
The aim is a model that understands and edits DNA natively, and can write working DNA that nature never wrote, in time including new gene therapies.
How it learns. Every design is checked against the sequence it produced, so what passes can teach the next round.
- DesignPair DNA with a precise instruction.
- GenerateProduce an edited sequence.
- CheckScore the exact edit and every constraint from the sequence itself.
- LearnKeep what passed, and what failed, for the next round.
A schematic of intent, not data. Helicase 1 is in development and has no published results.
What Helicase 1 is, and what is still to prove